Featured Publication
Cryo-electron microscopy of the f1 filamentous phage reveals insights into viral infection and assembly.
Conners, R.; León-Quezada, R.I.; McLaren, M.; Bennett, N.J.; Daum, B.; Rakonjac, J. & Gold, V.A.M.
Nature Communications (2023).
Phages are viruses that infect bacteria and dominate every ecosystem on our planet. As well as impacting microbial ecology, physiology and evolution, phages are exploited as tools in molecular biology and biotechnology. This is particularly true for the Ff (f1, fd or M13) phages, which represent a widely distributed group of filamentous viruses. Over nearly five decades, Ffs have seen an extraordinary range of applications, yet the complete structure of the phage capsid and consequently the mechanisms of infection and assembly remain largely mysterious. In this work, we use cryo-electron microscopy and a highly efficient system for production of short Ff-derived nanorods to determine a structure of a filamentous virus including the tips. We show that structure combined with mutagenesis can identify phage domains that are important in bacterial attack and for release of new progeny, allowing new models to be proposed for the phage lifecycle.
All Publications
The consequence of ATP synthase dimer angle on mitochondrial morphology studied by cryo-electron tomography.
Buzzard, E.; McLaren, M.; Bragoszewski, P.; Brancaccio, A.; Ford, H. C.; Daum, B.; Kuwabara, P.; Collinson, I. & Gold, V.A.M*. Biochem J. Accepted. (2024).
Structure of the two-component S-layer of the archaeon Sulfolobus acidocaldarius.
Gambelli, L.; McLaren, M.; Conners, R.; Sanders, K.; Gaines, M. C.; Clark, L.; Gold, V.A.M., Kattnig, D.; Mateusz; S.; Hanus, C.; Isupov, M. N.& Daum, B. eLife Accpeted (2024).
CryoEM reveals that ribosomes in microsporidian spores are locked in a dimeric hibernating state.
McLaren, M.; Conners, R.; Isupov, M.N.; Gil-Díez, P.; Gambelli, L.; Gold, V. A. M.; Walter, A.; Connell, S. R.; Williams, B. & Daum, B. Nat Microbiol (2023).
doi: 10.1038/s41564-023-01469-w
Structure, Biology, and Applications of Filamentous Bacteriophages.
Rakonjac, J. Gold, V.A.M, León-Quezada, R.I & Davenport, C. H. Cold Spring Harb Protoc (2023).
A restrictor complex of ZC3H4, WDR82, and ARS2 integrates with PNUTS to control unproductive transcription.
Estell, C., Davidson, L., Eaton, J. D., Kimura, H., Gold, V. A. M & West, S. Mol Cell (2023)
doi: 10.1016/j.molcel.2023.05.029
Cryo-electron microscopy of the f1 filamentous phage reveals insights into viral infection and assembly. Conners, R., León-Quezada, R.I., McLaren, M., Bennett, N.J., Daum, B., Rakonjac, J. & Gold, V.A.M. Nat Commun (2023).
doi: 10.1038/s41467-023-37915-w
Phage Therapy and The Public: Increasing Awareness Essential To Widespread Use.
McCammon, S., Makarovs, K., Banducci, S. & Gold, V.A.M. PLOS One (2023).
doi: 10.1371/journal.pone.0285824
Interaction of the periplasmic chaperone SurA with the inner membrane protein secretion (Sec) machinery.
Troman, L., Alvira, S.; Daum, B., Gold V. A. M. & Collinson, I. Biochem J (2023).
doi: 10.1042/bcj20220480
Electron cryo-microscopy reveals the structure of the archaeal thread filament
Gaines, M.C., Isupov, M. N. l, Sivabalasarma, S., Haque, R., McLaren, M., Mollat, C. M., Tripp, P., Neuhaus, A., Gold, V. A. M., Albers, S-V. & Daum, B. Nat Commun (2022).
doi: 10.1038/s41467-022-34652-4
An archaellum filament composed of two alternating subunits.
Gambelli, L., Isupov, M.N., Conners, R., McLaren, M.; Bellack, A.; Gold, V. A. M.; Rachel, R. & Daum, B. Nat Commun (2022).
doi: 10.1038/s41467-022-28337-1
CryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage
Conners, R., McLaren, M., Łapińska, U., Sanders, K., Stone, M. R. L., Blaskovich, M. A. T., Pagliara, S., Daum, B., Rakonjac, J. & Gold, V. A. M. Nat Commun (2021).
doi: 10.1038/s41467-021-26610-3
Maintenance of complex I and its supercomplexes by NDUF-11 is essential for mitochondrial structure, function and health
Knapp-Wilson, A., Pereira, G. C., Buzzard, E., Richardson, A., Corey, R. A., Neal, C., Verkade, P., Halestrap, A., Gold, V. A. M., Kuwabara, P. and & Collinson, I. J Cell Sci (2021)
doi: 10.1242/jcs.258399
Inter-membrane association of the Sec and BAM translocons for bacterial outer-membrane biogenesis
Alvira, S., Watkins, D. W., Troman, L., Allen, W. J., Lorriman, J. S., Degliesposti, G., Cohen, E. J., Beeby, M., Daum, B., Gold, V. A. M., Skehel, M. J. & Collinson, I. eLife (2020).
doi: 10.7554/eLife.60669
Cryo-electron microscopy reveals two distinct type IV pili assembled by the same bacterium
Neuhaus, A., Muniyandi, S., Salzer, R., Langer, J. D., Kruse, K., Sanders, K., Daum, B., Averhoff, B. & Gold, V. A. M. Nat Commun (2020).
doi: 10.1038/s41467-020-15650-w
Architecture and modular assembly of S-layers revealed by electron cryo-tomography
Gambelli, L., Meyer, B., McLaren, M., Sanders, K., Quax, T. E. F., Gold, V. A. M., Albers, S. & Daum, B. Proc Natl Acad Sci USA (2019).
Twitch or swim: towards the understanding of prokaryotic motion based on the type IV pilus blueprint
Daum, B.* & Gold, V. A. M.* Biol Chem (2018).
*joint corresponding authors
The ER morphology-regulating lunapark protein induces the formation of stacked bilayer discs
Wang, S., Powers, R. E., Gold, V. A. M. & Rapoport, T. A. Life Sci Alliance (2018).
Visualization of cytosolic ribosomes on the surface of mitochondria by electron cryo-tomography
Gold, V. A. M.*, Chroscicki, P., Bragoszewski, P. & Chacinska, A.* EMBO Rep (2017).
*joint corresponding authors
Analysis of mitochondrial membrane protein complexes by electron cryo-tomography
Gold V. A. M., Brandt, T., Cavellini, L., Cohen, M. M., Ieva, R. & van der Laan, M. Methods Mol Biol (2017).
doi: 10.1007/978-1-4939-6824-4_19
Recent progress in structure and dynamics of dual-membrane-spanning bacterial nanomachines
Gold V. A. M.* & Kudryashev, M.* Curr Opin Struct Biol (2016).
doi: 10.1016/j.sbi.2016.03.001
*corresponding authors
Topology and structure/function correlation of ring and gate forming domains in a dynamic secretin complex.
Salzer, R., D’Imprima, E., Gold V. A. M., Rose, I., Drechsler, M., Vonck, J. & Averhoff, B. J Biol Chem (2016).
Protein import by the mitochondrial presequence translocase in the absence of a membrane potential
Turakhiya, U., von der Malsburg, K., Gold V. A. M., van der Laan, M. & Ieva, R. J Mol Bio (2016).
doi: 10.1016/j.jmb.2016.01.020
Structure of the type IV pilus machinery in the open and closed state.
Gold, V. A. M. *, Salzer, R., Averhoff, B. & Kühlbrandt, W. eLife (2015).
doi: 10.7554/eLife.07380
*corresponding author
Visualizing active membrane protein complexes by electron cryotomography
Gold, V. A. M.*, Ieva, R., Walter, A., van der Laan, M., Pfanner, N. & Kühlbrandt, W. Nat Commun (2014).
doi: 10.1038/ncomms5129
*corresponding author
Visualization of ATP synthase dimers in mitochondria by electron cryo-tomography
Davies, K. M., Daum, B., Gold, V. A. M., Mühleip, A. W., Brandt, T., Blum, T. B., Mills, D. J. & Kühlbrandt, K. J Vis Exp (2014).
doi: 10.3791/51228
Membrane protein insertion and PMF-driven protein export by the bacterial holo-translocon SecYEG-SecDF-YajC-YidC
Schulze, R. J., Komar, J., Botte, M., Whitehouse, S., Gold, V. A. M., Huard, K., Berger, I., Schaffitzel, C. & Collinson, I. Proc Natl Acad Sci USA (2014).
A cell model for the initial phase of sporadic Alzheimer’s disease
Stockburger, C., Gold, V. A. M., Pallas, T., Kolesova, N., Miano, D., Leuner, K. & Müller, W. E. J Alz Dis (2014).
doi: 10.3233/JAD-140381
The dynamic action of SecA during the initiation of protein translocation
Gold, V. A. M., Whitehouse, S., Robson, A. & Collinson, I. J Biol Chem (2013).
doi: 10.7554/eLife.35112
Mobility of the SecA 2-helix-finger is not essential for polypeptide translocation via the SecYEG complex
Whitehouse, S., Gold, V. A. M., Sessions, R. B. & Collinson, I. J Cell Biol (2012).
Structure of the SecY complex unlocked by a preprotein mimic
Hizlan, D., Robson, A., Whitehouse, S., Gold, V. A. M., Vonck, J., Mills, D. J., Kühlbrandt, W. & Collinson, I. Cell Rep (2012).
doi: 10.1016/j.celrep.2011.11.003
The oligomeric state and arrangement of the active bacterial translocon
Deville, K.,# .Gold, V. A. M.,# Robson, A., Sessions, R. B., Baldwin, S., Radford, S. & Collinson, I. J Biol Chem (2011).
#equal first author contribution
The action of cardiolipin on the bacterial translocon
Gold, V. A. M., Robson, A., Bao, H., Romantsov, T., Duong, F. & Collinson, I. Proc Natl Acad Sci USA (2010).
Energy transduction in protein transport and the ATP hydrolytic cycle of SecA
Robson, A.,# Gold, V. A. M.# Hodson, S., Clarke, A. R., & Collinson, I. Proc Natl Acad Sci USA (2009).
#equal first author contribution.
A large conformational change couples the ATP binding site of SecA to the SecY protein channel
Robson, A., Booth, A. E., Gold V. A. M, Clarke, A. R. & Collinson, I. J Mol Biol (2007).
doi: 10.1016/j.jmb.2007.09.086
Structure and function of the bacterial Sec translocon
Gold V. A. M., Duong, F. & Collinson I. Mol Membr Biol. (2007).
doi: 10.1080/09687680701416570
Allosteric regulation of SecA: magnesium-mediated control of conformation and activity
Gold V. A. M., Robson, A., Clarke, A. R. & Collinson, I. J Biol Chem (2007).
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